City: Nashville, TN, US
Employer Reference: 10006561
Company Description
At Vanderbilt University , our work - regardless of title or role - is in service to an important and noble mission in which every member of our community serves in advancing knowledge and transforming lives on a daily basis. Located in Nashville, Tennessee, on a 330+ acre campus and arboretum dating back to 1873, Vanderbilt is proud to have been named as one of “America’s Best Large Employers” as well as a top employer in Tennessee and the Nashville metropolitan area by Forbes for several years running. We welcome those who are interested in learning and growing professionally with an employer that strives to create, foster and sustain opportunities as an employer of choice
We understand you have a choice when choosing where to work and pursue a career. We understand you are unique and have a story. We want to hear it. We encourage you to apply today so that you might become a part of our story.
Vanderbilt University is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran, or any other characteristic protected by law.
Job Description
The Research Assistant is part of the Chen lab in the Department of Molecular Physiology & Biophysics (MPB) at Vanderbilt University and is responsible for assisting the Principal Investigator in various aspects of research and management. The research responsibilities of this position include but not limited to the generation and maintenance of epigenetically modified cancer cell lines. The managerial responsibilities include lab organization.
The Chen lab consists of a culturally diverse team of postdoctoral fellows, graduate students, and Research assistants. The lab is very collaborative both within and outside. It is part of MPB and the Vanderbilt Diabetes Research and Training Center.
Duties and Responsibilities
Generation and maintenance of epigenetically modified cancer cell lines
- Maintain various cancer cell lines.
- Design and construct sgRNA expression vectors.
- Transfection and lentivirus production.
- Identify and characterize epigenetically modified cells by bisulfite conversion, PCR, qRT-PCR, and sequencing.
Lab Management
- Ordering and maintenance of research supplies.
- Lab organization
- Biosafety and chemical safety compliance.
Qualifications
A Bachelor's degree in Biology or related fields is preferred
Strong organizational/record keeping/problem solving preferred
The ability to follow instructions and to work independently within a team.
Experience with molecular techniques and cell culture.
Experience with microinjection and/or microscopic imaging is a plus.